Evaluation of activity of erythrocyte pyrimidine 5'-nucleotidase (P5N) in lead exposed workers: with focus on the effect on hemoglobin.
نویسندگان
چکیده
Anemia that accompanies lead poisoning is in part the result of various inhibitory effects of lead on heme biosynthesis. Lead also increases the rate of red blood cell destruction due to the profoundly depressed activities of erythrocyte pyrimidine 5'-nucleotidase (P5N) activities. We studied parameters of the two metabolic pathways in the workers exposed to lead to evaluate P5N in the lead exposed workers and which pathway has an effect on hemoglobin (Hb) level. 29 male workers in the secondary lead smelting as high exposure group, 46 male workers in the manufacturer of inorganic pigment as low exposure group and 56 clerical male workers from another plant as non-exposed group were studied. Activity of P5N, lead concentration in whole blood (PbB), zinc protoporphyrin (ZPP), Hb, and ferritin were determined. In the present study, P5N activity of nucleotide metabolic pathway correlated with Hb after controlling indices of iron deficiency anemia (ferritin) occurring concurrently and heme biosynthetic pathway (ZPP) in the high exposure group while heme biosynthetic pathway did not correlate with Hb after controlling other two variables in exposure groups. These findings suggest that P5N rather than heme biosynthetic pathway has a major effect on Hb level even in workers without manifest hemolytic anemia.
منابع مشابه
Evaluation of lead exposure in workers at a lead-acid battery factory in Korea: with focus on activity of erythrocyte pyrimidine 5'-nucleotidase (P5N).
OBJECTIVE To evaluate lead exposure among lead-acid battery workers in Korea, to evaluate in more detail the erythrocyte pyrimidine 5'-nucleotidase (P5N) test for lead exposure, and to evaluate the abnormal accumulation of erythrocyte pyrimidine nucleotides in the battery workers. METHODS Activity of P5N and other biological variables were examined in 66 exposed workers in a lead-acid battery...
متن کاملResponse of erythrocyte pyrimidine 5'-nucleotidase (P5N) activity in workers exposed to lead.
متن کامل
A simplified method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N) activity by HPLC and its value in monitoring lead exposure.
The method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N EC 3.1.3.5) activity has been simplified using an automated high performance liquid chromatograph (HPLC). The activity determined by the simplified method agreed closely with that obtained by conventional methods. In 161 lead workers P5N activity declined linearly with increasing blood lead concentrations (Pb-B) between 20 a...
متن کاملSimple determination of erythrocyte pyrimidine 5'-nucleotidase activity in human blood by high-performance liquid chromatography.
We developed a method for the determination of erythrocyte pyrimidine 5'-nucleotidase (P5N) activity in human blood using a high-performance liquid chromatograph (HPLC). In this method, UMP (uridine 5'-monophosphate disodium salt) was used as a substrate and assay solutions were directly prepared from whole blood. Uridine formed by the enzyme reaction of P5N was determined spectrophotometricall...
متن کاملDetermination of pyrimidine 5'-nucleotidase (P5N) activity in whole blood as an index of lead exposure.
A simple method for determining pyrimidine 5'-nucleotidase (P5N) activity in whole blood has been developed, inhibiting the plasma activity for UMP-hydrolysis by concanavalin (Con A). Con A specifically inhibits the activity of plasma 5'-nucleotidase (5N) but does not affect erythrocyte P5N activity. The anticoagulant EDTA partially inhibits 5N activity but slightly activates P5N. P5N activity ...
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عنوان ژورنال:
- Industrial health
دوره 40 1 شماره
صفحات -
تاریخ انتشار 2002